Transphosphatidylation by Phospholipase D
نویسندگان
چکیده
منابع مشابه
Synthesis of Metal Complex Modified Phospholipids by Phospholipase D-Catalyzed Transphosphatidylation
The synthesis of ferrocenyl-functionalized phospholipid was achieved through a biphasic transphosphatidylation reaction catalyzed by phospholipase D. Thus, the 1,2-distearoyl-sn-glycero3-phosphocholine headgroup was modified with hydroxymethylferrocene. 1,2-distearoyl-sn-glycero-3phosphocholine was chosen because it bears a choline group required for successful phospholipase D-catalysis. The us...
متن کاملSynthesis of novel phospholipids that bind phenylalkanols and hydroquinone via phospholipase D-catalyzed transphosphatidylation.
Phenylalkanols such as tyrosol and hydroxytyrosol (h-tyrosol), which possess antioxidant and anticancer properties, were phosphatidylated by phospholipase D (PLD)-catalyzed transphosphatidylation. After a 24-hour reaction of phosphatidylcholine (PC) and tyrosol with PLD, a new product was detected by TLC and identified to phosphatidyl-tyrosol by high-resolution MS and NMR analyses. The optimum ...
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BACKGROUND Phospholipases D (PLD) are major components of signalling pathways in plant responses to some stresses and hormones. The product of PLD activity is phosphatidic acid (PA). PAs with different acyl chains do not have the same protein targets, so to understand the signalling role of PLD it is essential to analyze the composition of its PA products in the presence and absence of an elici...
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The activity of bacterial phospholipase D (PLD), a Ca-dependent enzyme, toward phosphatidylcholine bilayers was enhanced 7-fold by incorporation of 10 mol % phosphatidic acid (PA) in the vesicle bilayer. Addition of other negatively charged lipids such as phosphatidylinositol, phosphatidylmethanol, and oleic acid either inhibited or had no effect on enzyme activity. Only negatively charged lipi...
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In the present study, we investigated the ability of an immobilized phospholipase A1 (PLA1) in degumming of soybean oil. The enzyme was immobilized by simple adsorption on bentonite without any further modification. The free and immobilized PLA1 were characterized by Fourier Transform InfraRed (FT-IR) spectroscopy and X-Ray Diffraction (XRD). The immobilizat...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1967
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)96298-8